Anti-TTF1 antibody [EP1584Y] ab76013 from Abcam

Product Specs
ItemAnti-TTF1 antibody [EP1584Y]
CompanyAbcam
Price Supplier Page View Supplier Product Page
Catalog Numberab76013
Antibody TypePrimary
ApplicationsImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
CloneEP1584Y
HostRabbit
Quantity10 UL, 100 UL
ClonalityMonoclonal
IsotypeIgG
Conjugate/TagUnconjugated
Gene NameNKX2-1
ReactivityHuman, Mouse, Rat
TargetTTF1

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) USP13 drives lung squamous cell carcinoma by switching lung club cell lineage plasticityMol CancerDecember 13, 2023Juntae Kwon, Jinmin Zhang, Boram Mok, Samuel Allsup, Chul Kim, Jeffrey Toretsky, Cecil HanCat#SK-4100). The primary antibodies include: USP13 (Santa Cruz, Cat# sc-514416) 1:600, NKX2-1 (Abcam, Cat# ab76013) 1:2000, SPC (Abcam, Cat# ab211326) 1:500, SOX2 (CST, Cat# 3728) 1:400, Cytokeratin 5 (Thermo Fisher Scientific

(2) Prime editing-mediated correction of the CFTR W1282X mutation in iPSCs and derived airway epithelial cellsPLOS ONENovember 29, 2023Chao Li, Zhong Liu, Justin Anderson, Zhongyu Liu, Liping Tang, Yao Li, Ning Peng, Jianguo Chen, Xueming Liu, Lianwu Fu, et al.used were as followings: SOX2 (09–0024, ReproCell), FOXA2 (sc-101060, Santa Cruz Biotech), NKX2.1 (ab76013, Abcam), ZO-1 (MA3-39100-A647, Thermo Scientific), MUC5B (HPA008246, Sigma-Aldrich), α-Tubulin (ab11315,

(3) SUCLG2 Regulates Mitochondrial Dysfunction through Succinylation in Lung AdenocarcinomaAdvanced ScienceOctober 30, 2023Qifan Hu, Jing Xu, Lei Wang, Yi Yuan, Ruiguang Luo, Mingxi Gan, Keru Wang, Tao Zhao, Yawen Wang, Tianyu Han, et al.for IHC. The tissue sections from tumors were stained with anti‐Ki67 (Abcam, ab15580), anti‐TTF1 (Abcam, ab76013), and anti‐SUCLG2 antibodies. Micrographs were obtained using an Olympus IX71 microscope. mtDNA Extraction

(4) Epigenetic reprogramming of a distal developmental enhancer cluster drives SOX2 overexpression in breast and lung adenocarcinomaNucleic Acids ResearchOctober 27, 2023Luis E Abatti, Patricia Lado-Fernández, Linh Huynh, Manuel Collado, Michael M Hoffman, Jennifer A Mitchellmin, followed by overnight incubation at 4°C in a primary antibody cocktail (rabbit anti-NKX2.1, Abcam ab76013 at 1:200; rat anti-SOX2, Thermo Fisher Scientific 14–9811-80 at 1:100). After washes with TBS-T, sections

(5) Novel Mouse Cell Lines and In Vivo Models for Human High-Grade Neuroendocrine Lung Carcinoma, Small Cell Lung Carcinoma (SCLC), and Large Cell Neuroendocrine Carcinoma (LCNEC)International Journal of Molecular SciencesOctober 18, 2023Enrique Recuero, Sara Lázaro, Corina Lorz, Ana Belén Enguita, Ramón Garcia-Escudero, Mirentxu SantosCambridge, UK); 1/2000 dilution of anti-CGRP (C8198, MilliporeSigma); 1/200 dilution of anti-TTF1 (ab76013, Abcam, Cambridge, UK); 1/50 dilution of anti-p63 (ab53039-100, Abcam, Cambridge, UK); and 1/100 dilution

(6) Generation of 3D lung organoids from human induced pluripotent stem cells for modeling of lung development and viral infectionHeliyonSeptember 1, 2023Shashi Kant Tiwari, Tariq M. Ranaanti-prosurfactant protein C (proSP-C),Millipore,AB3786 Rabbit anti-RAGE,Abcam,ab216329 Mouse anti-Hop (E−1),Santa Cruz,Sc-398703 Rabbit anti-NKX2.1,Abcam,ab76013 Rabbit anti-SOX9,Millipore,AB5535 Rabbit anti-ECAD,Cell Signaling Technology,3195 Mouse anti-CC10

(7) Multiple synchronous primary malignant neoplasms: A case report and literature reviewOncology LettersAugust 17, 2023Linlin Hao, Lihui Zhang, Chuanjie Xu, Min Jiang, Geyi Zhu, Jie Guo,Cell Signaling,3625,1:3000 Anti-BRG1,Abcam,ab110641,1:100 Anti-GATA3,Abcam,ab199428,1:500 INI-1 (MRQ-27) Mouse monoclonal,Sigma-Aldrich; Merck KGaA,272M-1,1:200 Anti-TTF1,Abcam,ab76013,1:250 p53 Rabbit pAb,ABclonal,A0263,1:50 Primary antibodies.

(8) Sirtuin1-Mediated Deacetylation of Hypothalamic TTF-1 Contributes to the Energy Deficiency ResponseInt J Mol SciAugust 7, 2023Dasol Kang, Hye Rim Yang, Dong Hee Kim, Kwang Kon Kim, Bora Jeong, Byong Seo Park, Jeong Woo Park, Jae Geun Kim, Byung Ju Leeserum albumin in Tris-buffered saline–tween buffer and then incubated with rabbit anti-TTF-1 antibody (#ab76013, Abcam, Inc., Cambridge, UK; 1:1000 dilution), rabbit anti-Sirt1 antibody (Sigma-Aldrich, St. Louis,

(9) An invasive zone in human liver cancer identified by Stereo-seq promotes hepatocyte-tumor cell crosstalk, local immunosuppression and tumor progressionCell researchAugust 1, 2023Wu L, Yan J, Bai Y, Chen F, Zou X, Xu J, Huang A, Hou L, Zhong Y, Jing Z, et al.(#ab225732, Abcam), FPR1 (#ab113531, Abcam), S100P (#ab124743, Abcam), CK20 (#13063 S, Abcam), TTF1 (#ab76013, Abcam), PanCK (#CST4545, CST), and p53 (#CST2527S, CST). The multiplexed IF standard operation procedures

(10) STUB1-mediated ubiquitination regulates the stability of GLUD1 in lung adenocarcinomaiScienceJuly 21, 2023Hu Q, Lei J, Cheng Z, Xu J, Wang L, Yuan Y, Gan M, Wang Y, Xie Y, Yao L, et al.company for immunohistochemistry. Anti-Ki67, anti-TTF1 antibodies were purchased from Abcam (ab15580 and ab76013, #9662). Micrographs were obtained using Olympus IX71 microscope. Quantification and statistical analysis Statistical

(11) Role of GLIS3 in thyroid development and in the regulation of gene expression in thyroid specific Glis3KO miceResearch squareJuly 7, 2023Kang HS, Grimm SA, Liao XH, Jetten AM.GFP (A-11122, Life Technology or GFP-1010, Aves Labs), PAX8 (NBP1-32440, Novus Biologicals), NKX2.1 (ab76013, Abcam), CDH16 (PA5-57589, ThermoFisher), ZO-1 (61-7300, ThermoFisher), E-Cadherin (CDH1; U3254, Sigma-Aldrich),

(12) Development of a novel air–liquid interface airway tissue equivalent model for in vitro respiratory modeling studiesScientific ReportsJune 22, 2023Timothy Leach, Uma Gandhi, Kimberly D. Reeves, Kristina Stumpf, Kenichi Okuda, Frank C. Marini, Stephen J. Walker, Richard Boucher, Jeannie Chan, Laura A. Cox, et al.anti-MUC5B antibody (1:2000; University of North Carolina, Chapel Hill), anti-TP63 antibody (1:300; ab76013; Abcam), anti-acetylated α-tubulin antibody (1:5000; T7451; Sigma). Multispectral IHC staining was performed

(13) Live-cell invasive phenotyping uncovers the ALK2/BMP6 iron homeostasis pathway as a therapeutic vulnerability in LKB1-mutant lung cancerbioRxiv : the preprint server for biologyJune 14, 2023Koo J, Seong CS, Parker RE, Dwivedi B, Arthur RA, Dinasarapu AR, Johnston HR, Claussen H, Tucker-Burden C, Ramalingam SS, et al.(ab192303), Transferrin receptor (ab84036), SCD1 (ab236868), Ferritin (ab75973), EpCAM (ab71916) and TTF1 (ab76013) antibodies were from Abcam. KRAS (sc-30), p53 (sc-126 for human), a-Tubulin (sc-8035) antibodies were

(14) Differentiation of human induced pluripotent stem cells into functional lung alveolar epithelial cells in 3D dynamic cultureFrontiers in Bioengineering and BiotechnologyJune 14, 2023Sarah Alsobaie, Tamador Alsobaie, Amal Alshammary, Sakis MantalarisSanta Cruz,1:50–1:500 FOXA2,Goat polyclonal IgG,sc-6554; Santa Cruz,1:50–1:500 TTF,Rabbit monoclonal IgG,AB76013; Abcam,"1:100–1:1,000" List of primary antibodies for immunocytochemistry staining. Abbreviations:

(15) Directed differentiation of mouse pluripotent stem cells into functional lung-specific mesenchymeNature communicationsJune 13, 2023Alber AB, Marquez HA, Ma L, Kwong G, Thapa BR, Villacorta-Martin C, Lindstrom-Vautrin J, Bawa P, Wang F, Luo Y, et al.anti-proSFTPC, Abcam ab211326, 1/250; rabbit anti-SOX9, Abcam ab185966, 1/500; rabbit anti-TTF1 (Nkx2-1), Abcam ab76013, 1/500; rabbit anti-p63, Cell Signaling 13109, 1/500, goat anti-RFP, MyBioSource, MBS448122, 1/500)

(16) Regulating the cell shift of endothelial cell-like myofibroblasts in pulmonary fibrosisThe European respiratory journalJune 1, 2023Wu X, Zhang D, Qiao X, Zhang L, Cai X, Ji J, Ma JA, Zhao Y, Belperio JA, Boström KI, et al.previously [44]. We used specific antibodies to CD34 (553731; BD Bioscience or ab54208; Abcam), Nkx2.1 (ab76013; Abcam) and VE-cadherin (562243; BD Biosciences). The nuclei were stained with 4′,6-diamidino-2-phenylindole

(17) Sleep-wake patterns are altered with age, Prdm13 signaling in the DMH, and diet restriction in miceLife science allianceJune 1, 2023Tsuji S, Brace CS, Yao R, Tanie Y, Tada H, Rensing N, Mizuno S, Almunia J, Kong Y, Nakamura K, et al.immunofluorescent staining, samples were stained using primary antibodies: anti-Nkx2-1 (TTF-1) (1:500, ab76013; Abcam) and secondary antibodies. To stain cFos, samples were stained with anti-cFos (1:1,000, 226003;

(18) Atf3 defines a population of pulmonary endothelial cells essential for lung regenerationeLifeMay 26, 2023Niethamer TK, Levin LI, Morley MP, Babu A, Zhou S, Morrisey EE.,Santa Cruz Biotechnology,sc-398703 RRID:AB_2687966,1:100 Antibody,NKX2-1 (TTF1) (rabbit monoclonal),Abcam,ab76013 RRID:AB_1310784,1:50 Antibody,PDPN (mouse monoclonal),Abcam,ab10288 RRID:AB_297027,1:100 Antibody,CD45-PerCP-Cy5.5

(19) Transcription factor NKX2–1 drives serine and glycine synthesis addiction in cancerBritish Journal of CancerMay 11, 2023Elien Heylen, Paulien Verstraete, Linde Van Aerschot, Shauni L. Geeraerts, Tom Venken, Kalina Timcheva, David Nittner, Jelle Verbeeck, Jonathan Royaert, Marion Gijbels, et al.SimpleChIP Plus Sonication Chromatin IP Kit (Cell Signaling) was used to perform all ChIP experiments using either anti-TTF1 (Abcam, ab76013) or anti-IgG (Cell Signaling, #2729) antibodies according to manufacturer’s instructions using Dynabeads

(20) Organoid modeling reveals the tumorigenic potential of the alveolar progenitor cell stateResearch SquareApril 13, 2023Jingyun Kim, Susanna M. Dang, Susanna Schurmann, Antonella F.M. Schurmann, Antonella Dost, Margherita Paschini, Margherita Bhetariya, Preetida Bhetariya, Shannan J. Ho Bronson, Shannan Ho Sui(Thermo Scientific, 14-0441-82, 1:500), YFP (Abcam, ab6673, 1:400), SPC (Abcam, ab211326, 1:1,000), Nkx2-1 (Abcam, ab76013, 1:250), Hmga2 (GeneTex, GTX629478, 1:200). The primary antibodies were diluted in blocking buffer and

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Anti-TTF1 antibody [EP1584Y] from Abcam

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Anti-TTF1 antibody [EP1584Y] from Abcam

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